Translational_Unit

Part:BBa_K2230002:Design

Designed by: Yi-Lun Huang   Group: iGEM17_Mingdao   (2017-10-19)


RBS-EmR/pSB1A2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No


Source

EmR (Erythromycin resistance gene) was amplified from pMG36e vector and cut by XbaI & PstI. Then, the fragment was assembled with RBS/pSB1A3 (BBa_B0034) cut by SpeI & PstI

References