Translational_Unit
Part:BBa_K2230002:Design
Designed by: Yi-Lun Huang Group: iGEM17_Mingdao (2017-10-19)
RBS-EmR/pSB1A2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
No
Source
EmR (Erythromycin resistance gene) was amplified from pMG36e vector and cut by XbaI & PstI. Then, the fragment was assembled with RBS/pSB1A3 (BBa_B0034) cut by SpeI & PstI